Description

Kligler Iron Agar (KIA) is a medium used for Enterobacteriacae differentiation (confirmatory test), based on their ability to ferment sugars, such as glucose and lactose, and to produce hydrogen sulfide.

Sugar fermentation is detected by the medium's yellowing, while the production of H₂S is detected by the medium's blackening. The phenol red is present as a pH indicator, instead ammonium citrate iron is present as an indicator of H₂S.

The KIA has an identical TSI medium composition, with the exception that the TSI contains, in addition to glucose and lactose, also sucrose. The KIA agar is arranged to be on sloped; with the upper portion exposed to oxygen (aerobic chamber), while the lower portion (bottom) is oxygen-free (anaerobic chamber).

Packing:

• Cod. B.70.094 Pack. 5 Kg
• Cod. 70.094 Pack. 500 g
• Cod. 71.094 Pack. 100 g
• Cod. 18.0102 Pack. 10 test-tubes on sloped, ready to use

Bibliography:

• ISO 21528-2:2004 (NF V08-039-2) Microbiology of food and animal feeding stuffs -- Horizontal methods for the detection and enumeration of Enterobacteriaceae -- Part 2: Colony-count method
• ISO/TS 21872-2: 2007 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. - Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae