Description

Phosphate Buffered Saline (PBS) is primarily used for suspending cells from monolayer cultures on glass. The cells growing in a monolayer on a glass surface can be suspended by a number of methods, including the application of chelating agents, such as diamino-ethane-tetra-acetic acid (Sodium Versenate 0.02% wt /v), which bind divalent cations (calcium and magnesium ions), allowing the cells to detach from the glass. The PBS solution is then used to rinse the cell sheet free of growth medium prior to application of the chelating agent.

Packing:

• Cod. 70.906 Pack. 500 g
• Cod. 22.021 Pack. 6 bottles 100 ml
• Cod. 22.109 Pack. 6 bottles 200 ml

Bibliography:

• Dulbecco e Vogt (1954) J. Esp. Med . 99. 167-182.
• Paul J. (1965) "Cell and Tissue Culture" 3a ed. Livingstone Ltd., Londra .
• Sambrook, Fritsch e Maniatis (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, volume 3, appendice B.12