Description

SABOURAUD DEXTROSE BROTH WITH CHLORAMPHENICOL is a medium suitable for the isolation of opportunistic pathogens fungi, such as Aspergillus, Fusarium and Mucor from clinical samples highly contaminated by mixed microbial flora such as urine, aspirates bronchial, sputum, bronchial washing. Furthermore, it is also used for the isolation of pathogenic fungi that are sensitive to cycloheximide such as Criptococcus Neoformans.

This medium is extremely selective both for the low value of pH (5.6), and for the presence of chloramphenicol.

Packing:

• Cod. H 70.459 Pack. 10 bags Hydrobag (1 litre preparation each)
• Cod. 70.459 Pack. 500 g
• Cod. 71.459 Pack. 100 g
• Cod. 18.0051 Pack. 10 test-tubes, ready to use

Bibliography:

• NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-2nd ed.; Approved Standard.
• APHA (1963) - Diagnostic Procedures and Reagents.
• ISO 7954:1987 Microbiology -- General guidance for enumeration of yeasts and moulds -- Colony count technique at 25° C-
• NF V 08-059. Novembre 2002 (3ème tirage d’Août 2003). Microbiologie des aliments. Dénombrement des levures etmoisissures par comptage des colonies à 25 °C. Méthode de routine.
• NCCLS document M22-A3, 2004. Quality Assurance for Commercially prepared Microbiological Culture Media.-3nd ed.; Approved Standard.
• XP CEN ISO/TS 11133-2 (V 08-104-2). Janvier 2004. Microbiologie des aliments. Guide pour la préparation et laproduction des milieux de culture. Partie 2 : Guide général pour les essais de performance des milieux de culture.
• ISO 6611 / FIL 94. Octobre 2004. Lait et produits laitiers. Dénombrement des unités formant colonie de levures et/oumoisissures. Comptage des colonies à 25 °C.
• IRA – Convegno Maggio 2000 – La Purezza delle materie prime e dei prodotti finite nell’industria cosmetica: Verifica e metodiche per la valutazione della contaminazione microbiologica del prodotto finito
• UNI EN ISO 21148: 2009 Cosmetici _ Microbiologia – Istruzioni generali per l’analisi microbiologica
• UNI EN ISO 18416:2009 Cosmetici _ Microbiologia – Ricerca della Candida albicans
• R. ISTISAN 07/5 Bonadonna L, Ottaviani M (Ed.). Metodi analitici di riferimento per le acque destinate al consumo umano ai sensi del DL.vo 31/2001. Metodi microbiologici. Roma: Istituto Superiore di Sanità; 2007- Met ISS A 016B Funghi