Description

The isolation and the cultivation of Brucella can be conducted by direct method, on agarized medium or indirect method, with pre-enrichment procedure.

De Miguel at Al. formulated the CITA agar (selective medium) in 2011, wich was particularly suitable for the research and isolation of the different species of Brucella. The new formulation as indicated in the recent revision (O.I.E. manual 2016 Chapter 2.1.4.), allows the development of all Brucella species, with satisfactory inhibition of contaminating microorganisms.

Sterilely, on the medium are added 2 vials of CITA Selective Supplement (Cod. 76.048) and 2 vials of sterile ram serum (Cod. 21.0168).

Packing:

• Cod. 70.207 Pack. 500g
• Cod. 14.0690V Pack. 20 plates Ø 90 mm, ready to use

Bibliography:

• M. J. De Miguel, C. M. Marín, P. M. Muñoz, L. Dieste, M. J. Grilló, and J. M. Blasco, “Development of a selective culture medium for primary isolation of the main Brucella Species,” Journal of Clinical Microbiology, vol. 49, no. 4, pp. 1458–1463, 2011
• T. di Febo, M. Luciani, O. Portanti, B. Bonfini, R. Lelli, and M. Tittarelli, “Development and evaluation of diagnostic tests for the serological diagnosis of Brucellosis in swine,” Veterinaria Italiana, vol. 48, no. 2, pp. 133–156, 2012
• BioMed Research International 10.04.2014 (Vol .2014_ Article ID 702072 9 - Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms
• Man. O.I.E._2016 Chapter 2.1.4 - Infection with Brucella abortus, B melitensis and B. suis.